cell aggregation process

Cell Aggregation an overview ScienceDirect Topics

Cell aggregation is a typical bioengineering method that aims to reconstitute bioengineered organ germ that will properly reproduce epithelial–mesenchymal interactions and organogenesis (Sharpe and Young, 2005; Yen and Sharpe, 2006).

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Cell Aggregation an overview ScienceDirect Topics

Cell aggregation is a typical bioengineering method that aims to reconstitute bioengineered organ germ that will properly reproduce epithelial–mesenchymal interactions and organogenesis (Sharpe and Young, 2005; Yen and Sharpe, 2006).

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Sponge cell aggregation PubMed

Dissociated cells form functional aggregates during a process which can be sub-divided into three phases: first, formation of small primary aggregates in the presence of Ca2+; second, formation of secondary aggregates in the presence of an aggregation factor and third, reconstitution of a functional system of water-containing channels by rearrangement in the secondary aggregates.

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Controlled cell aggregation in a pulsed acoustic field

2012-9-1 · Controlling the aggregation process. The aggregation process starts when isolated particles in levitation converge towards a specific point, where the acoustic energy is maximal. In order to generate the transversal migration, the resonance frequency must be carefully tuned.

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Cell Aggregation Assays SpringerLink

Cell aggregation assays have been set up to test the functionality of the complex in epithelioid tumor cells. Functional integrity of the complex is a prerequisite for cell-cell adhesion between epithelial cells, and measuring cell aggregation in vitro has thus become another elegant tool to study differences between invasive and noninvasive cell types.

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Sponge cell reaggregation: Mechanisms and

2014-7-22 · It considers the behavior of dissociated cells in suspension, the mechanisms and factors involved in reaggregation, and the rate and stages of this process in

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Dynamical analysis of antibody aggregation in the

2013-12-4 · Although secreted antibody is known to aggregate during cell culture process [ 1, 2 ], the underlying mechanism is still poorly understood due to the lack of observation of the aggregation process. We applied the TRPA column to dynamical aggregation analysis of Ex3-scDb-Fc in CHO cell culture.

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Identification of process conditions influencing

Finally, the aggregation reducing conditions were verified and applied to another production system using a different bioprocess medium and another CHO cell line producing another mAb. Our results show that protein aggregation can be controlled during cell culture and helps to improve bioprocessing of mAbs, by giving insights into the protein aggregation at its origin in mammalian cell culture.

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Identification of cell culture conditions to control

2012-1-1 · Factors that contribute to protein aggregation during the cell culture stage can be the cell itself, the culture environment (e.g., pH, temperature), or culture additives. With respect to the cell, folding of newly synthesized polypeptide chains requires the assistance of folding catalysts and molecular chaperones . Genetic manipulation during cell line development, such as vector construction and

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Cell Aggregation Parametric House

2 天前 · The design process followed different methods of expressing these aggregation structures, exploring the relationship between negative and positive. This resulted in two very different versions based on the same principle of association, one expressing the cells continuous surface, and another that starts from cell

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WikiGenes Cell Aggregation

Biological context of Cell Aggregation. Expression of the Drosophila cell adhesion molecule neuroglian in S2 cells leads to cell aggregation and the intracellular recruitment of ankyrin to cell contact sites [16]. The induction of tyrosine phosphorylation through ICAM-3 and LFA-1 antigens was studied by immunofluorescence, and it was found that

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[Cell-surface Initial Aggregation Process of Amyloid-β

It is widely accepted that oligomers are more neurotoxic than amyloid fibrils in the aqueous-phase aggregation of Aβ. Membrane-mediated amyloidogenesis is also relevan [Cell-surface Initial Aggregation Process of Amyloid-β Peptides Studied by Fluorescence Correlation Spectroscopy] Yakugaku Zasshi. 2021;141(6) :825-829.

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Cell Aggregation Assays SpringerLink

Cell aggregation assays have been set up to test the functionality of the complex in epithelioid tumor cells. Functional integrity of the complex is a prerequisite for cell-cell adhesion between epithelial cells, and measuring cell aggregation in vitro has thus become another elegant tool to study differences between invasive and noninvasive

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Red Blood Cell Aggregation and Dissociation in Shear

However, the aggregation process was not investigated and the cell deformation was less evident in Liu et al. (2004). The small cell deformation could be due to the finite element modeling of the RBC membrane as a thin shell, in which the experimentally measured

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Controlled cell aggregation in a pulsed acoustic field

2012-9-1 · For cell aggregation, different experimental parameters were varied including the cell concentration, number of pulses, repetition time and voltage. The typical cell size was 20 μm and the typical velocities were 223 μm/s in continuous mode and 147 μm/s in pulsed mode, corresponding to acoustic forces of ∼12 × 10 −11 N and ∼5.5 × 10

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Protein aggregation and bioprocessing PubMed

Protein aggregation is a common issue encountered during manufacture of biotherapeutics. It is possible to influence the amount of aggregate produced during the cell culture and purification process by carefully controlling the environment (eg, media components) and implementing appropriate strategies to minimize the extent of aggregation.

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Role of Ionic Strength in Staphylococcal Cell

2021-3-12 · Cell aggregation plays a key role in biofilm formation and pathogenesis of Staphylococcus species. Although the molecular basis of aggregation in Staphylococci has already been extensively investigated, the influence of environmental factors, such as ionic strength, remains poorly understood. In this paper, we report a new type of cellular aggregation of Staphylococci that depends solely on

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Cellular strategies for controlling protein aggregation

2010-10-14 · The aggregation of misfolded proteins is associated with the perturbation of cellular function and ageing. However, protein aggregation can also be a regulated process that deposits aggregates at

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Measuring the aggregation of CHO cells prior to

2017-5-27 · Measuring the aggregation of CHO cells prior to single cell cloning allows a more accurate determination of the probability of clonality. Kerensa J. Klottrup, Corresponding Author. The manufacturing process for biotherapeutics is closely

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